Contact: Dr. Christos Chinopoulos MD, PhD
A Kinetic Assay of Mitochondrial ADP-ATP Exchange Rate Mediated by the Adenine Nucleotide Translocase

The present website outlines the methodology for measuring mitochondrial ADP-ATP exchange rate mediated by the adenine nucleotide translocase (ANT),  originally published in:

Biophys J. 2009 Mar 18;96(6):2490-504. doi: 10.1016/j.bpj.2008.12.3915

entitled "A Novel Kinetic Assay of Mitochondrial ATP-ADP Exchange Rate Mediated by the ANT"
by Christos Chinopoulos, Szilvia Vajda, László Csanády, Miklós Mándi, Katalin Mathe and Vera Adam-Vizi.

The method can be applied not only in isolated mitochondria, but also permeabilized cells and tissue homogenates.

An improved calibration step of this method has been published in Methods Enzymol. 2014;542:333-48.

Follow the links appearing on the left, starting from the top. The 'core' of the method is outlined in the Buffers, chemicals, Kds determination of ADP, ATP to Mg2+, and Conversion of [Mg2+]free to [ATP]. However, it is advisable to go through all of the links, if you wish to understand what you will be actually doing.

At the bottom of the website you can also find a link pointing to work related to ANT1 as a voltage sensor of the mitochondrial permeability transition.
MCQs Biochem semi final